Inhibition of dimethylebenz (a) anthracene (dmba) or croton oil induced skin tumorigenesis in swiss albino mice by aloe vera treatment
Authors:Int J Biol Med Res. 2011; 2(3): 671-678
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In the present study, anti-cancer property of Aloe vera was evaluated against 7,12-dimethyl benz(a)anthracene (DMBA) induced skin tumorigenesis in Swiss albino mice. A single topical application of 7,12-dimethyl benz(a)anthracene (100 μg/100 μl of acetone), followed 2 weeks later by repeated application of croton oil (1% in acetone three times a week) for 16 weeks exhibited 100 percent tumor incidence (group III).
In contrast, animals treated topically with Aloe gel (group IV) or orally with Aloe extract (group V) and topical with Aloe gel + orally with extract (group VI) exhibited 40, 50 and 20 per cent tumor incidence, which significantly higher than 100% tumor incidence in the group III (control). The cumulative number of papillomas during the observation period of 16 weeks was significantly decreased in the Aloe treated groups IV, V and VI (4, 5 and 2 in Aloe gel, Aloe extract, and Aloe gel + Aloe extract treated animals respectively) in compare to 36 cumulative number of papillomas in carcinogen control group.
The average latent period significantly increased from 4.9 weeks in the control group to 5.3, 6.4 and 6.5 weeks in all Aloe treated groups. The tumor burden and tumor yield were significantly lesser (1.33, 1.25 and 1.0 and 0.4, 0.5 and 0.2) as compared to DMBA/ croton oil treated control (3.6 and 3.6).
Furthermore, the level of lipid peroxidation was significantly lesser than in the control animals (group III) in skin. In addition, depleted levels of reduced glutathione (GSH), DNA, catalase and protein were restored in Aloe-treated groups. The study has revealed the inhibition of dimethylebenz (a) anthracene (DMBA)/croton oil induced skin tumorigenesis in Swiss albino mice by Aloe vera treatment.