Protective effect of coumarin on cell surface glycoconjugates abnormalities during 7,12-dimethylbenz(a)anthracene (DMBA) induced oral carcinogenesis

Authors:Nagarethinam Baskaran, Shanmugam Manoharan, Simon Silvan, Duraisamy Palanimuthu,Duraisamy Rajasekaran, Murugaraj Manoj Prabhakar, Sekar Karthikeyan, Ramachandran Srinivasan and Shamsul Afaq Wani
Int J Biol Med Res. 2011; 2(3): 643-647  |  PDF File

Abstract

Aims: The major aims of the present study was to focus the protective effect of coumarin on cell surface glycoconjugates abnormalities during 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Methods: DMBA painting in the hamster buccal pouch three times per week for 14 weeks developed oral squamous cell carcinoma. The levels of glycoconjugates (protein bound hexose, hexosamine, sialic acid and fucose) were analyzed by using specific colorimetric methods. Results: An increase in glycoconjugates was noticed in the plasma and buccal mucosa of oral cancer bearing golden Syrian hamsters. Coumarin administration orally at a dose of 100mg/kg b.w to hamsters treated with DMBA brought back the status of glycoconjugates to near normal range. Conclusion: Present study thus focused the protective effect of coumarin on cell surface abnormalities occurring during DMBA-induced hamster buccal pouch carcinogenesis.