Prevalence of uncommitted antigens and lymphoid markers in acute myeloid leukemia
Authors:Int J Biol Med Res. 2012; 3(3): 1931 - 1934.
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Flow cytometry is a well known investigation for detecting the cluster of differentiation (CD antigens) in leukemic cells. It has the capability in a short time to measure multiple parameters on each single cell, which is sufficient to diagnose types, subtypes and even estimate to some the future prognoses.
Objective: To evaluate Prevalence of uncommitted antigens and lymphoid markers in acute myeloid Leukemia flow cytometric results for a patient’s diagnosis of acute myeloid leukemia.
Method and material: Results of flow cytometry for 163 patients, between January 2006 and December 2011 who were newly diagnosed as having acute myeloid leukemia (AML) were retrospectively revised, 110 patients were male and 53 patients were female with male to female ratio close to 2: 1. And age distribution as 35 pediatric patients in comparison to 128 adult patients.
Most of patients were adult (128/ out of 163 patients (78.5%), and 110/163 (67.5%) were males. Among all AML Among all AML results showed 6patients M0(3.7%),32 patients M1 (19.6%), 47 patients M2 (28.8%), 15 patients M3 (9.2%), 38 patients M4 (23.3%),15 patients M5(9.2%),4 patients M6(2.5%), and 6 patients (3.7%)M7.
In our study 6.2% of AML pts showed negative for myeloperoxidase, CD34 negative 48/141 pts (34%), and CD117 negative 21/142 pts (14.8%). while CD7 was positive in 28/130 (21.5%).
• Flow cytometry is an informative test in diagnosis and identify AML subtypes.
• Having single uncommitted Antigens may suggest less differentiation and
• Positive lymphoid Antigens marker (especially CD7) in AML patients do not change their diagnosis, although make them more badly prognosis phenotypically.
• Negative myeloperoxidase is not against diagnosis of AML.