Green synthesis of silver nanoparticles by using leaf and stem of manilkara zapota l. and antioxidant and antidiabetic activity

Abstract

Nanoparticles possess unique biological properties. Determination of in vitro antioxidant activity was carried out using DPPH assay and Reducing power assay and in vitro antidiabetic activity using Alpha amylase assay of AgNPs leaf and stem of Manilkara zapota L. Chacterization was carried out using UV-Vis spectroscopy and Scanning electron microscopy. In investigation it was found that AgNPs leaf and stem are stable and round to spherical in shape. In DPPH assay IC50 value for leaf and stem was determined to be 124.62+0.007μg/ml and183.43+0.007μg/ml respectively. In reducing power assay results shows that at 10 μg/ml concentration of test sample reducing power is 74.64% for leaf, 69.88% for stem and 71.28% for ascorbic acid. In Alpha amylase inhibitory assay the IC50 value was determined to be 25.94+0.002 μg/ml for leaf 51.78+0.003μg/ml for stem. Results revealed that the plant parts of Manilkara zapota L. have potent antioxidant and antidiabetic property.