Effect of salivaryadenectomy of the pregnant mother on testicular lactase dehydrogenase in mice

Authors:BODARE R. D., PILLAI M. M.
Int J Biol Med Res. 2012; 3(4): 2560-2564  |  PDF File

Abstract

The large body of evidences indicate that epidermal growth factor (EGF) is synthesized, stored and secreted by the submandibular glands and also by the sublingual glands. EGF shows stimulating activity on various epidermal epithelial tissues both in vitro and in vivo. A series of observations suggest that the growth factors and in particular EGF plays an important role in the male reproductive system. The concentration of EGF in the submandibular gland and plasma of the female mice increases substantially during pregnancy. The midgestational sialoadenectomy of female mice reduces EGF level. To show the effect of salivariadenectomy of the pregnant mother, on the development and function of testis, testicular lactase dehydrogenase isoenzyme was studied biochemically and electrophoretically. LDH-X is one of the LDH isoenzymes which is unique to pachytene spermatocytes, spermatids and mature sperm. The pregnant females were salivariadenectomised (sublingualectomy, sialoadenectomy and both sublingualectomy + sialoadenectomy) at 10th day of their gestation period. The 20 days, 45 days, 60 days old male offsprings of salivaridenectomised mother as well as male offsprings of the same age of sham operated mother were sacrificed by the cervical dislocation and their testis were used to study the electrophoretic separation of lactate dehydrogenase and estimation of lactate dehydrogenase. In the testis of 20 days, 45 days, 60 days normal mice of sham operated mother, the LDH was separated into six bands i.e. I, II, III, IV, V and LDH-X. But in the testis of above aged mice of sublingualctomised, sialoadenectomised and salivariadenectomised mother, LDH-X band was disappeared. The LDH activity was decreased significantly in the testis of 20 days, 45 days, 60 days offsprings of sublingualectomised mother as compared to normal mice of sham operated mother. The above results suggest that maternal EGF affects the development and functions of testis.