Renin- angiotensin- aldosterone system gene polymorphisms in type 2 diabetic patients among the mewari population of rajasthan

Authors:Pulakes Purkait, Prem Chand Suthar, Vijay Kumar Purohit, J. M. Naidu, B.N. Sarkar
Int J Biol Med Res. 2013; 4(2): 3128-3134  |  PDF File

Abstract

Aim: Diabetes is the one of the most serious health problems of modern times and India is the top most country with the highest number of individuals affected with type 2diabetes and an extremely limited data on the genetics of diabetes in the Indian populations is available. In this context the aim of the present study was to investigate the distribution of RAAS pathway genes polymorphism and its relationship with Type 2 Diabetes patients in Mewari population. Methods: The present study examined the prevalence of RAAS gene polymorphism among 111 Mewari individuals from Udaipur, Rajasthan, India. Among them 50 individuals belong to type 2 diabetic (T2DM) and 61 healthy controls. Isolated DNA samples from the studied subjects were genotyped using PCR-RFLP methods. Result: There was a significant difference in age, gender, SBP, BMI, glucose, GGT, urea, creatinine, LDL and total protein between T2DM and Control subjects (p <0.05). The minor allele frequency of RAAS pathway gene polymorphism AGT (rs4762), REN (rs41317140), ACE (rs4646994), AGTR1 (rs5186), CYP11B2 (rs1799998) in T2DM patients (T2DM) are found to be 0.18, 0.20, 0.41, 0.05 and 0.30 respectively. The allele frequencies of the same polymorphisms among the normal controls are found to be 0.13, 0.16, 0.48, 0.10 and 0.40 respectively. The general associations were not found in significant result (p> 0.05). Conclusion: The study found that no association of RAAS gene polymorphisms with increased risk of type 2 diabetes among the Mewari population except rs4762. The AGT (rs4762) was associated at recessive model (p = 0.038).