A survey of antimicrobial susceptibility patterns of bacterial isolates from community acquired significant bacteriuria among pre-school children in a municipality in calabar

Authors:Godwin T. Jombo, Gyuse AN, Odey F, Ibor S, Bolarin DM , Utsalo SJ , Denen Akaa P , Okwori EE
Int J Biol Med Res. 2010; 1(4): 172-176  |  PDF File

Abstract

Urinary tract infection is often not assumed to be a common clinical finding among children by clinicians. The study was therefore set up to ascertain the antimicrobial susceptibility patterns of bacteria recovered from urine of pre-school children with significant bacteriuria. Pre-school children attending nursery schools from five distinct geographical locations in Calabar municipality were recruited using random sampling methods. Mid-stream urine samples were obtained, transported, stored and processed using standard laboratory procedures, while antimicrobial susceptibility tests were carried out using modified Kirby-Bauer’s disk diffusion methods. Other information such as age, sex, parents or guardians occupation were obtained using structured questionnaires; other anthropometric measurements such as weight and height were also taken. Data was analysed using Epi Info 6 statistical software. The prevalence of significant bacteriuria was 7.3% with no significant gender difference (P> 0.05). The commonest organisms isolated were Escherichia coli 36.4%, Proteus mirabilis 21.2%, and Klebsiella pneumoniae 18.2%, others were Pseudomonas aeruginosa (9.1%), Enterobacter species (6.0%) and Staphylococcus aureus (9.1%). All the isolates of Pseudomonas aeruginosa and 66.7% of Klebsiella pneumoniae were from males (P< 0.05) and 76.7% of Staphylococcus aureus from females (P< 0.05). Antibiotics with highest activity were: ceftriaxone (100%), cefuroxime (33%-100%), nalidixic acid (50-100%), and gentamicin (50%-100%); those with least activity were ampicillin (0%), co-trimoxazole (0%-33%) and augmentin (0%-17%). Urinary tract infection could occur in pre-school children with out obvious clinical features, hence surveillance through routine urine screening is recommended. Ceftriaxone, cefuroxime, nalidixic acid and gentamicin may be considered in the absence of a sensitivity report.