Preliminary test on the effectiveness of protein fraction of aedes aegypti larvae against bacteria growth of escherichia coli

Authors:Andi Ernawati , Syahribulan, Mashuri Masri
Int J Biol Med Res. 2014; 5(3): 4197-4202  |  PDF File

Abstract

Background & Objectives: Aedes aegypti is the main vector of dengue and chikungunya diseases. This mosquito is known to have proteins and fats that play a role in the process of life. This study aims to determine the fraction of protein and fat content of Aedes aegypti larvae and its ability to inhibit the growth of Escherichia coli bacteria. Methods: Fractionation of protein with ammonium sulfate at levels of 0-30%, 30-40%, 40-60%, and 60-80%. Determination of protein content by Lowry method and fat by standard laboratory operations. Test of effectiveness by measuring the diameter of inhibition zone around the paper disc. Result: The results of protein content measurements using 0.2 ml with 10 times dilution showed that 30-40 % of fraction contains highest levels of total protein 5.26 mg and lowest in the 0-30 % fraction 3.76 mg. The results of protein content measurements using 0.02 ml of sample extract with 100 times dilution showed that 40-60 % of fraction contains the highest total protein content 27.03 mg and lowest in the 0-30% fraction 4.88 mg. Fat content is only found in the protein fraction of 0-30% and 30-40 %. Result of effectiveness test showed that protein fraction of 30-40% and 60-80 % are bakteriocide. Fraction of 30-40 % has 0.6 % fat content and the fraction of 60-80 % have no fat content. Crude extract, fractions of 0-30 %, and 40-60 % are bacteriostatic. Protein fraction of 0-30 % has fat content of 0.8%, while the crude extract and fractions of 40-60 % do not have fat content. Interpretation & Conclusion: Protein fraction of Ae.aegypti showed inhibition to the growth of E. coli. Fat content does not give effect to bacteriostatic or bacteriocide of bioactive compounds of Ae.aegypti.