Protective effect of cocoa extract on albino mice skin tumor after exposure to 7,12 dimethylbenz () antrasena (dmba) and 12-o-tetradecanoylphorbol-13-acetate (tpa) malondyaldehyde (mda) analysis and histopathology

Authors:S Wahab, MN Massi, A Gemini, K.Djawad
Int J Biol Med Res. 2014; 5(4): 4648-4653  |  PDF File

Abstract

Aims. Oxidative stress result in DNA destruction in the induction cells. If this damage is irreversible, DNA will undergo mutation and may lead to cancer. One of cancer caused chemical and often use in experimental study is DMBA/TPA. Thus the chemical is utilized in this study to examine protective effect of cocoa extract on mice. Method. 30 albino mice, age 6-9 weeks, weight 20-30 gr were used as sampel study. The animal were divided into 6 groups with different treatment; group I, is negative control which receive no treatment; group II is control group receiving DMBA/TPA; group III receiving aceton, and group IV,V, and VI each receiving cocoa extract of 200,400 and 800 ppm, respectively. Group II-VI expose to DMBA three times a week with every other day fashion in week 1, followed by application of 4µg TPA on week 2-12. All the animal were sacrificed 24 h after last exposure and samples were taken and proccess for MDA and histopathology examination. Result. MDA level in 400 and 800 ppm cocoa extract groups were higher than control, while it is lower in 200 ppm group than in control. Anova result showed no signicant differences among control and cocoa groups (p> 0.05). Histopathology examination result on DMBA/TPA control, aceton and 200 ppm cocoa groups consist of severe dysplasia and squamous cell carcinoma, whereas in 400 and 800 ppm cocoa groups some samples exhibit mild dysplasia. Kruskall Wallis statistical analysis indicates significant differences among control and cocoa groups (p≤ 0.05) Conclusion.Topically applied 400 and 800 ppm cocoa exhibit protective effect on cancer formation with the best protection effect shown in concentration of 800 ppm.