Principle and techniques of immunohistochemistry – a review

Authors:Arpita Kabiraj, Jagriti Gupta, Tanya Khaitan, Preeti Tomar Bhattacharya
Int J Biol Med Res. 2015; 6(3): 5204-5210  |  PDF File

Abstract

Immunohistochemistry is a technique for identifying cellular or tissue constituents (antigens) by means of antigen-antibody interactions, the site of antibody binding being identified by direct labelling of the antibody, or by use of secondary labelling method. Although histological analysis of Haematoxylin & Eosin stained tissue sections remains at the core of the practice of head and neck surgical pathology, immunohistochemistry has become a powerful tool in the armamentarium of the pathologist. The selection of antibodies for the immunohistochemical testing is made on the basis of their tumor specificity and the likelihood that they will react with the tumor under evaluation. IHC is a well-established ancillary technique to facilitate the diagnosis of infectious and neoplastic processes in animals. Immunohistochemical studies have traditionally focused on markers of specific cell and/or tumour type as aids in the diagnosis of specific tumours.