Patterns of serum protein electrophoresis, our experience at king hussein medical center, jordan

Authors:Mansour al-hiary, MD; Asim Momani, MD; Rame Khasawneh, MD; Bayan Zghoul, MD; Lana Habahbeh, MD Abdel razaq wreikat, MD; Sanaa saidat,MD
Int J Biol Med Res. 2015; 6(1): 4702-4704  |  PDF File

Abstract

Background: The aim of this study is to analyze the results of serum protein electrophoresis, to determine the electrophoretic patterns of separated proteins. Materials and Methods: This study was conducted at the Immunology Department at Princess Iman Center for research and Laboratory Sciences, Royal Medical Services- Amman/Jordan during November and December, 2013. Data was collected after analysis of 54 specimens of patients who attended outpatient clinics at King Hussein Medical Center. All specimens were analyzed by gel electrophoresis. Results: 54 specimens were included in this study with an average age between 30 & 85 years. 28 (51.8%) were males and 26 (48.2%) were females. Analysis of densitometry diagram and agaroe gel electrophoresis revealed normal electrophoretic pattern in 7 patients (13%), acute inflammation in 8 patients (15%), chronic inflammation in32 patients (59%) and monoclonal gammopathy in 7 patients (13%).For monoclonal gammopathy cases, immunofixation was done to identify heavy and light chains. 4 samples identified as IgG ⱪ, 2 samples IgA ⱪ and 1 sample IgA λ. Conclusion: Different patterns of serum protein electrophoresis were encountered and the spectrum extends from acute and chronic inflammation to monoclonal gammopathy. Therefore serum protein electrophoresis is the first step in differentiating benign from malignant conditions.