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Study on nonfermenting gram negative bacteria from various clinical samples in a tertiary care hospital

Authors:Kamalraj M, Sivashankari S, Thamarai S, Apurba shankarsastry
Int J Biol Med Res. 2015; 6(4): 5230-5235  |  PDF File


Non fermenting gram negative bacilli are a group of heterogenous, aerobic, non sporing bacteria. they are saprophyte in nature and are also found as commensals in man and animals. Hence this study aims to isolation, identification, and antibiotic susceptibility for non fermenting gram negative bacilli from clinical samples and to find out the clinical significance among the inpatient in meenakshi medical college and hospital kanchipuram. A total of 103 non fermenters isolated from clinical sample such as pus, sputum, urine, blood and ear swab were included in the study. All these were identified by a battery of test as per standard laboratory technique. Antimicrobial sensitivity testing was performed for all isolates by kirby bauer disc diffusion method. In these non fermenters, Pseudomonas were 83.5% followed by Acinetobacter 15.5% species was the second commonest non-fermenter followed by shewanella sp.,(0.9%). The NF GNBs are known to cause of variety of nosocomial infection. Resistance pattern among the nosocomial bacterial pathogen may vary widely from place to place even within the same country over time. These nonfermenters are isolated from clinical specimens frequently and are resistant to most of the routinely used antibiotics showing that organisms need to be taken seriously and identified not just regarded as contaminants.