Platelet count; automated vs manual estimation on blood smear prince rashid hospital, rms

Authors:Asim Momani, Rame Khasawneh, Mansour Hyari, Bayan Zgoul, Hala Sokhnee, Ngah Drous, Mohamamd maaytah
Int J Biol Med Res. 2015; 6(3): 5148-5150  |  PDF File

Abstract

Objective: The objective of this study is to evaluate the accuracy of manual platelet count estimates by comparing the results of platelet count using the automated counter with the manual method (the use the stained thin blood film) for the same sample at the same time in the Hematology Dept. at PRH Clinical Laboratory. Material and Methods: This is a cross-sectional study, which was conducted at PRH, where we randomly selected blood specimens of patients presenting to the Clinical Laboratory during August through October 2013. The specimens were processed by the automated method and the manual method simultaneously. Results: The manual method for performing platelet count estimates on blood smears gives estimates that are not significantly different from the counts by the automated method on the Sysmex KX21 automated counter at p < 0.05. Despite that platelet count estimates with the manual method in general are slightly higher than the automated method; it is a reliable technique and appears to provide platelet count estimates to use in quality assurance. Conclusion: The study concludes that the traditional method of estimating platelet counts from blood smears to evaluate automated results appears to provide adequate quality assurance.