Application of cytochemical staining in the diagnosis and classification of acute and chronic leukemia

Authors:SabaAmiri Kojuri, MohammadFaezi Ghasemi, NasimAmiri Kojuri, Ali Raefi, Saman Ayoubi
Int J Biol Med Res. 2017; 8(1): 5834-5842  |  PDF File

Abstract

Background: Lymphoblastic leukemia based on cell morphology dominant coloring Cytochemical into three main groups: 1L, 2L, 3L classify, Although the clinical value of the expected lifespan in adults is unknown, but in terms of prognosis and clinical course and response to treatment methods in children is important. Methods: In the 109 peripheral blood samples from patients suspected of Razi Hospital Hematology Center was developed leukemia, and follows Cytochemical staining was performed on each sample. Results: In 19 samples by alpha-naphthalen propanoic acetate esterase staining positive 15/78 percent and 84/21 percent were negative. Of the 12 samples analyzed by acid phosphatase staining was found that 25% of the samples were positive and 75% negative, also the 25 samples that were analyzed by alkaline phosphatase staining, 100% of the negative and none of the samples were positive, and 34 samples were examined by staining Periodic acid shifts 97/55% of the negative samples and only 2/94 percent positive, and from 12 samples by staining was evaluated Naphthile AS-D 16/66% positive and 83/33% of the samples were negative. Conclusion: The diagnosis of leukemia, the disease situation and select pin for the treatment of all cases with morphological characteristics of the cells was not possible, therefore, to reach the correct diagnosis Cytochemical tests and immune cell phenotype and cytogenetic studies are needed.