Purification and characterization of setaria equina antigen and evaluate its efficacy for diagnosis of human filariasis

Authors:Ibrahim Rabia, Manal Ahmed, Mona Zohery , Faten Nagy, Amany Rady, Noha Mahana, Tarek Diab, Azza El Amir
Int J Biol Med Res. 2017; 8(1): 5851-5856  |  PDF File

Abstract

Lymphatic filariasis (LF) is still a public health problem in tropical and subtropical countries. The most common causative agents of human filariasis are Wuchereria (W.) bancrofti, Brugia (B.) malayi and B .timori. This work was designed to evaluate the diagnostic efficacy of 3 types of antigens including crude Setaria (S.) equina, purified S. equina and Dirofilaria (D.) immitis antigens in detecting anti-W. bancrofti IgG polyclonal antibody (pAb) in W. bancrofti infected patients .Crude S. equina antigen from S. equina adult worms was purified by DEAE sephadex G-50 ion exchange chromatography and gel filtration chromatography on Sephadex-G-200 HR column. A total of 92 Egyptian individuals were enrolled in this study and divided into 3 groups. Group I included 42 patients infected with W. bancrofti, group II included 30 patients infected with other parasites as a parasitic control group (10 patients with Schistosomiasis mansoni, 10 patients with Fasciolasis and 10 patients with Hydatidosis ) and group III as healthy controls (n= 20). Detection of anti-W. bancrofti IgG pAb in patientís sera by indirect ELISA using crude S. equina, purified S. equina and D. immitis antigens showed 78.6 %, 85.71% and 80.95% sensitivity rates and 68%, 76% and 80% specificity rates, respectively. In conclusion, purified S. equina antigen is promising for diagnosis of W. bancrofti in human. Further study is highly recommended in order to obtain monoclonal antibody against purified fractions of S. equina adult worm antigen to reach higher specificity and sensitivity.