The relationships between sorbitol dehydrogenase (sdh) level and diabetic rethinopathy in diabetes melitus type-2 patients

Authors:Ramzi Amin, Reyno Satria Ali, Rachmat Hidayat, Hikmat Permana, Arief S Kartasasmita, Dany Hilmanto
Int J Biol Med Res. 2017; 8(3): 6020-6022  |  PDF File

Abstract

Background The conversion of sorbitol accumulation to fructose by SDH triggers the osmotic damage in retinal endothelial cells and pericyte through the activation of advanced glycation end products (AGEs), oxidative-nitrosative stress, Protein Kinase C (PKC) pathway activation, inflammation, and the imbalance of growth factor. The osmotic damage eventually leads to DR (Diabetic Rethinopathy). Methods This is a case-control study. DM type 2 patients who had the direct ophthalmoscopy and fundus imaging in dr. Mohammad Hoesin Central General Hospital opthalmology outpatient in Palembang was selected as a subject in this study. Patient’s blood sample was collected from a median cubital vein for 3 mL and stored in ethylene diamine tetraacetic acid (EDTA) coated tube for SDH assay by ELISA. Results The average level of SDH in DR subjects was 13,3 ±7,8 ng/mLand the average level of SDH in Non DR was 10,7±2,3 ng/mL, p=0,044. There was no significant difference in subjects with DR and subjects in control group in level of SDH by chi square-test, which the level of SDH was devided into two groups, higher than 11,18 ng/mL and lower than 11,18 ng/mL by ROC curve. Conclusion There was significant difference in subjects with DR and subjects in control group in level of SDH. The average of SDH level in DR subjects were more higher than Non DR subjects.