Effect of centella asiatica extracts on shigella dysenteriae and bacillus coagulans sp. compared to commonly used antibiotics.

Abstract

Centella asiatica is known for its antibacterial property from time immemorial. A comparative study on the action of its crude extract on Shigella dysenteriae and Bacillus coagulans was performed to see the difference of its effects on the two bacteria. As one of them is a pathogen and the other one is useful for human hosts. The study revealed that 1 mg of the extract dissolved in 1ml 4% DMSO inhibits Shigella. dysenteriae with zone of inhibition of 12±1 mm but could not act on Bacillus coagulans in same concentration. However, known antibiotics act on both the organisms. The action of acetone, chloroform and methanol extracts of Centella asiatica on Shigella dysenteriae did not vary significantly. The GC-MS analysis of the methanol extracts with peaks > 90% similarity index with NIST library revealed bicyclo[7.2.0]undec-4-ene,4,11,11-trimethyl-8-methylene; caryophyllene; 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester; 1h-3a,7-methanoazulene, octahydro-1,4,9,9-tetramethyl.