Bacterial infections in the dialysis

Authors:Sekhri-Arafa. N , Allag. H
Int J Biol Med Res. 2018; 9(1): 6187-6193  |  PDF File

Abstract

AIMS: Our study focused on bacterial infections in dialysis patients (under peritoneal dialysis or hemodialysis). Method: this study was carried out for six months, from January to June 2017 at the bacteriology laboratory of the renal clinic of Daksi of Constantine. The analysis was carried out on strains isolated from different pathological samples. The identification of bacteria was achieved by the conventional methods used in the laboratory, namely the study of their morphological, biochemical API cropping and galleries (analytical index profile). The detection of resistance phenotypes was studied by the conventional method of dissemination of antibiotic disks on medium agar Mueller Hinton and reading and interpretation criteria recommended by the Clinical and Laboratory Standard Institute (CLSI). Results: 200 cases of infectious were analyzed. Results showed that the majority of patients are treated by peritoneal dialysis with a higher frequency (63.80%) than those treated with hemodialysis (36, 19). 112 bacterial strains were isolated from various samples. Analysis of dialysate samples showed a clear predominance of Gram-positive Cocci (75%) versus Gram-negative bacilli (25%). First there are the staphylococci with a frequency of 62, 5% followed Enterobacteriaceae with a predominance of E. coli to 17, 85%, 12.5% of Streptococcus, and gram negative bacilli not fermenting with predominance of Pseudomonas aeruginosa, with a percentage of 7,14%. Conclusion: In this study we have identified the organisms responsible for infections in dialysis patients and their antibiotic resistance. The frequency of these infections is significant. The diagnosis of infections has identified the main germs in question. The isolated bacteria show resistance to several antibiotics tested which aggravates the situation of this immune- suppressed population and diminishes the therapeutic possibilities. The best treatment remains the prevention by the early detection of these infections. Compliance with hygiene measures, individual and collective cleanliness and maintenance of the hospital environment