Role of various hematological parameters in diagnosis of clinically suspected cases of neonatal septicemia

Authors:Gautam Chandrakoshi, Santosh Kumar Gond
Int J Biol Med Res. 2018; 9(3): 6389-6392  |  PDF File

Abstract

Aim: 1-To evaluate the various hematological parameters ,CRP, quantitative and qualitative changes in neutrophil and to identify causative organism of sepsis by blood culture . Method: To evaluate the hematological parameters we used 5 part cell counter and peripheral smear stained with leishman stain, for CRP was done by latex kit method and to identify the causative organism we did blood culture of 100 neonates admitted in NICU in Shyam shah medical college and associated Sanjay Gandhi hospital. Results: In my study out of 100 sample sensitivity of total WBC count is 51.7 %, specificity is 66.6%, positive predictive value is 68% and negative predictive value is 50% The Sensitivity of absolute neutrophil count is observed to be 70.6% and specificity is 85.7%. Positive predictive value of the test is 87.2% and negative predictive value is 68%. Sensitivity of I:TNR is 89.6%, specificity is 73.8%, positive predictive value is 82.5% and negative predictive value is 83.7%1. Sensitivity of platelet count is 48.2%, specificity is 80.9%, positive predictive value is 77.7% and negative predictive value is 53.1%. Sensitivity of CRP is 70.6%, specificity is 78.5%, positive predictive value is 82%and negative predictive value is 66%1. Sensitivity of morphological changes (presence of toxic granulation and dohle bodies) in neutrophils is 51%, specificity is 60.7%, positive predictive value is 55.5% and negative predictive value is 56.3%. In blood culture most common pathogenic organism grown is klebsiella followed by acinetobacter in our study.Conclusion:we concluded that in our study blood culture shows more sensitivity and specificity to diagnose neonatal septicemia but ittakes 48 to 72 hours to give result on the other hand TLC, ANC, I:TNR,Platelet count,CRP and morphological changes in neutrophils gives early result and altogether give better sensitivity and specificity to diagnose neonatal septicemia.