Enhanced anticancer activity of barleria longiflora l.f in human cervical cancer (hela) cells

Authors:BASKARAN. A, KARTHIKEYAN. V, SEBASTIAN RAJASEKARAN. C
Int J Biol Med Res. 2019; 10(1): 6613-6615  |  PDF File

Abstract

The objective of this study was to examine the cytotoxic effect of Barleria longiflora L.f stem extract on cervical cancer (HeLa) cell lines. HeLa cells were cultured in EMEM medium and incubated with different concentrations (12.5, 25, 50, 100 and 200 µg/ml) of ethanol extract of Barleria longiflora L.f. The method used for this research to determine cell viability was an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Barleria longiflora decreased cell viability in malignant cells in a concentration dependent manner. The IC50 value of Barleria longiflora extract was found to be 184.3 µg/ml. These data showed ethanolic extract of Barleria longiflora reduces the viability of cervical cell lines and may have potential as anti-cancer compounds.