Dna barcoding study on barleria acuminata ness an endemic medicinal plant of pachamalai hills, tiruchirappalli, tamil nadu, india.

Authors:V. Karthikeyan, A. Baskaran, C. Sebastian Rajasekaran
Int J Biol Med Res. 2019; 10(1): 6571-6575  |  PDF File

Abstract

Barleria acuminata Ness belongs to the family Acanthaceae which is endemic distribution. Presence of this plant in pachamalai hills a part of Eastern Ghats, located in Tiruchirappalli District of Tamil Nadu, India. The rbcL gene is widely used in systematic studies to resolve divergences at many taxonomic levels. The collected B. acuminata were subjected to sequence analysis of rbcL gene and the same was deposited in Gene Bank and an accession number has been assigned for the same (Accession No. KR337263). The data was further analyzed for the construction of neighbor joining tree and to infer the evolutionary divergence among the maximum identical sequences retrieved from NCBI Gene Bank through BLAST search. The results of the search revealed that the rbcL sequence of B. acuminata has not been sequenced earlier from any part of this world. It is obvious that the rbcL sequence of B. acuminata may be used for the identification of this species reported from any part of the world through BLAST analysis if the identical sequences are submitted to Gene Bank in future.