The morphological, anatomical and physiological aspects of maize plant after applying the different level of temperature treatments on pre-sowing maize seeds.

Authors:Zobia Anwer, Sidra Shabbir
Int J Biol Med Res. 2019; 10(1): 6576-6578  |  PDF File

Abstract

Research was conducted to study the morphological, physiological and anatomical aspects after different level of temperature treatments on pre-sowing maize seeds. Sample seeds were treated with 4 different levels of temperature stress viz. 0⁰C (T1), 6⁰C (T2), 35⁰C (T3) and 60⁰C (T4) respectively for 24 hrs. Experiment was performed in CBD with triplicates and sow at 35⁰C soil temperature. The germination percentage of vernalized seeds did not affect at 35⁰C because seeds were vernalized before sowing. The morphological characters like seedling length (root+shoot), No. of leaves, length of flag leaves, leaf area, fresh weight, dry weight, moisture content, yield, cob length and physiological parameters like chlorophyll content (a, b and carotene) and 17 selected parameters of anatomy under 3 magnification of microscope (4x, 10x, 40x) were studied after staining. Chlorophyll content studied with 2 extraction methods (i) 80% acetone (ii) Distilled H2O. Leaf extract centrifuge at 3000rpm for 10mins and studied with the help of AAS at different wavelength (chl a, 645nm; chl b, 663nm; carotene, 652nm). The chilling temperature treatment (0⁰C) of seeds extremely damages the plants due to crystal formation. At 60⁰C plants were largely affected because 66% moisture content of seeds decreased due to heat shock. Seeds which treated with both high and chilling temperature were shown the thin stem, root, low amount of chlorophyll content, lowest value of morphological parameters and narrow leaf after emergence but vernalized seeds were shown the antagonistic characters as compared to heat shock plants. The maize at 6⁰C had shown the fast emergence process and highest germination percentage.