In vitro anti-microbial and anticancer properties of ink gland from kalinga ornata(alder & hancock 1864).

Authors:Ramachandiran Sivaramakrishnana, Naidu Kavithaa, Muthuvel Arumugam
Int J Biol Med Res. 2019; 10(1): 6658-6663  |  PDF File

Abstract

The present study investigated the anticancer and antimicrobial properties of ink gland from Kalinga ornata using three different solvents such as acetone (AIG), ethyl acetate (EIG) and methanol (MIG). The ink gland sample was extracted with three different solvents and subjected to anti-microbial activities and then purified through CM-Sephadex, the pooled fraction was screened for anticancer activity and characterization was done using High-Performance Liquid Chromatography (HPLC) and Fourier-Transform Infrared Spectroscopy(FT-IR) respectively. The antimicrobial activity was conducted to determine the zone of inhibition against the human pathogen. Preliminarily, fractionations of the extracts were performed by CM-Sephadex (C-50) column chromatography. The purified fractions were pooled based on their high absorbance by UV-visible spectroscopy. Among the purified fractions, MIG showed significant anti-proliferative activity (41.06%) than AIG (30.44%) and EIG (14.35%) against colon cancer cell line (SW620). It is concluded that bioactive components from marine natural products pave way for finding a potential drug candidate.