Analysis of proteoglycan content in combined intermediate and deep zones of degenerating patellar articular cartilage in adult male mice after administration of diazepam

Authors:Anber Saleem, M Yunus Khan, Ruqqia Minhas
Int J Biol Med Res. 2019; 10(3): 6796-6799  |  PDF File

Abstract

Abstract: Objective: The basic purpose of this study was to analyze the effect of diazepam on the proteoglycan content of combined intermediate and deep zones of patellar articular cartilage in adult male mice. Study design: An experimental study. Place and duration of study: Department of Anatomy, College of Physicians and Surgeons CPSP, Regional center, Islamabad from June 2015- June 2016.Methodology: Animals were categorized into two main groups A control (mice not treated with diazepam) and B experimental groups (mice treated with diazepam) with further division of Group A into three subgroups A1 (mice without any intervention), A2 (mice kept immobilized for 30 days), A3 (mice kept immobilized for 30 days and then remobilized for next 30 days, treated with normal saline during remobilization period). Similarly, experimental group B mice were immobilized for initial 30 days and then remobilized for next 30 days and treated with diazepam during remobilization period. Group A1 was compared with group A2 to assess any change in proteoglycan content of combined zones of patellar articular cartilage due to immobilization. Group A2 was then compared with group A3 to analyze the effect of remobilization on proteoglycan content of combined zone. Finally, comparison between group A3 and group B was done to evaluate the effect of diazepam on proteoglycan content of combined zone remobilized mice. Qualitative analysis of staining grades of proteoglycan content in combined zones was done by chi square test using SPSS version 16. Conclusion: Diazepam administration does not have any impact on the proteoglycan content of combined zones of patellar articular cartilage in adult male mice.