Non-tuberculous mycobacteria identification in species level from respiratory specimens in south india

Authors:Kuppamuthu Ramakrishnan*, V.Thiyagarajan*, S.Vijayaraj* C.Ponnuraja*& R.Radhakrishnan *
Int J Biol Med Res. 2021; 12(1): 7204-7207  |  PDF File


ABSTRACT Non-tuberculous mycobacteria (NTM) is a term used for a huge number of potentially pathogenic and non-pathogenic environmental mycobacterial species other than Mycobacterium tuberculosis complex (MTBC) and Mycobacterium leprae. Aims and Objectives: To identify the species on NTM isolated from the sputum samples of the base line surveillance study (BS study) by high performance liquid chromatographic (HPLC) method. Methods: This was a base line surveillance study carried out from model DOTS programme implemented in Thiruvallur. Base line survey has been completed at the Government Hospital Thiruvallur, Chennai, during the period April 2009 to July 2011. The clinical evaluation of 98 sputum samples were collected and processed for culture by modified pertroff method. After culture selection criteria for non-mycobacteria were identified to species level using HPLC analysis were done by standard operating procedure. Results: A total of 795 cultures of NTM were undergone HPLC identification 98 among them M.terrrae was predominant 42 out of 92 followed by M.avium (12) , M.chitae (10), M.fortuitum (7), M.farcinogenes (6), M.simiae (5), M.fortuitum complex (4), M.bovis (3), M.flavescens (3), and the unidentified 6 was noticed. Conclusion: It concludes the rate of NTM from respiratory specimens at our area has been increasing steadily and therefore, early differentiation between pulmonary TB and NTM lung disease in patients with AFB smear-positive specimens is necessary. Additionally our study says patient presenting with features of TB is less likely to have NTM disease, in related settings.