Oxidant-antioxidant status, high sensitive C-reactive protein and homocysteine levels in type 2 diabetic patients with and without microalbuminuria

Authors:Palanisamy Pasupathi , Jawahar Farook , Palanisamy Chinnaswamy
Int J Biol Med Res. 2010; 1(3): 04 - 08  |  PDF File

Abstract

High levels of high sensitive C-reactive protein (hs-CRP), homocysteine (Hcy) and oxidative stress are known to be associated with premature vascular disease in type 2 diabetes mellitus (DM). The aim of this study was to estimate homocysteine levels and oxidant-antioxidant status and to determine the relationship between them in type 2 diabetic patients with and without microalbuminuria. The study population consisted of 75 subjects [age and sex-matched] divided into three groups. Fasting blood samples were obtained from 50 diabetic patients (25 with and 25 without microalbuminuria) and 25 healthy subjects. The level of blood glucose, HbA1C, urea, creatnine and serum lipids were significantly higher in the patients with microalbuminuria compared with patients without microalbuminuria and control subjects, comparatively higher level in patients with microalbuminuria. On the other hand HDL-C was found to be significantly lower in patients with microalbuminuria. The level of hs-CRP and Hcy levels were found to be significantly higher in patients with microalbuminuria compared with patients without microalbuminuria and healthy controls. The level of plasma thiobarbituric acid reactive substances (TBARS) was markedly increased and the level of enzymic and non-enzymic antioxidant was significantly decreased in the patients with microalbuminuria compared with patients without microalbuminuria and control subjects. The present study highlights the occurrence of lipid peroxidation and possible breakdown of antioxidant status in patients with microalbuminuria. Decreased antioxidant levels, increased lipid peroxidation and increased hs-CRP and Hcy levels were observed in patients with microalbuminuria.