Chemopreventive Effect of Phyllanthus niruri on DMBA Induced Skin Papillomagenesis in Swiss Albino Mice

Authors:Priyanka Sharma, Jyoti Parmar, Preeti Verma, Priyanka Sharma, P.K.Goyal
Int J Biol Med Res. 2010; 1(4): 158 – 164  |  PDF File

Abstract

Phyllanthus niruri is a useful medicinal weed for herbalist and it holds a reputed position in both Ayurvedic and Unani system of medicine. The aim of this work was to evaluate the chemopreventive effect of hydro-alcoholic extract of the whole plant of Phyllanthus niruri, in 7-9 weeks old male Swiss albino mice, on two stage process of skin carcinogenesis induced by a single topical application of 7, 12-dimethylbenz (a) anthracene (DMBA) and two weeks later promoted by repeated application of croton oil till the end of experiment (i.e. 16 weeks).The oral administration of P.niruri at 1000 mg/kg/b.wt./day during peri and post-initiational phases of papillomagenesis showed significant reduction in tumor incidence, tumor yield, tumor burden and cumulative number of papillomas as compared to carcinogen treated control. The average latent period significantly increased from 7.93 weeks in the control group to 10.52 weeks in the Phyllanthus niruri administrated group. Furthermore, a significant increase in reduced glutathione(p<0.001), catalase (p<0.001) and protein (p<0.001) level in skin was observed in the P.niruri administered groups as compared to carcinogen treated control, whereas MDA formation in lipid peroxidation was inhibited significantly by P.niruri extract (p<0.001). The results from the present study suggest the chemopreventive effect of Phyllanthus niruri in DMBA induced skin papillomagenesis in Swiss albino mice.