Comparative study of venous liquid blood and dried blood for hiv infection in infants

Authors:D.Anitha , P.Sumathi ,S.MiniJacob
Int J Biol Med Res. 2012; 3(1): 1369-1372  |  PDF File

Abstract

Polymerase Chain Reaction (PCR) is the only tool to identify the HIV status in infants less than 18 months of age. The objective of the study was to compare the results obtained from venous liquid blood on tubes and dried blood on two different filter papers for HIV infection in infant diagnosis. A total of 100 infant samples were collected on EDTA tubes and coated onto the filter papers. DNA was extracted from all these samples using liquid blood, whatman 903 and FTA cards. Nested PCR was performed using gag genome and the positive bands were visualized by gel electrophoresis. Of 100, seven infants were found to be HIV positive and 93 were HIV negative. The results obtained from two types of filter papers were compared with the venous blood and the sensitivity and specificity was 100%. Seven HIV positive infants and sixteen HIV negative infants were repeated with the freshly collected venous samples and also the samples directly coated onto the filter paper from heel prick, PCR was performed and the results were concordant. The blood collected onto the filter papers are ideal for HIV testing in infants in resource limited countries