Study of reduced glutathione in seminal plasma and spermatozoa nuclear chromatin decondensation test (ncdt) in human subjects with different fertility potential.

Authors:Shrikant Ashok Shete, Sandip Meghnad Hulke, Avinash Thakare, Prashant Patil
Int J Biol Med Res. 2012; 3(2): 1636 – 1639  |  PDF File

Abstract

The nuclear chromatin decondensation test (NCDT) detects the ability of the sperm chromatin to undergo decondensation following fertilization. It is known that there is positive relationship between concentrations of seminal plasma reduced glutathione and male fertility.Aims: In this study we try to evaluate correlation between seminal plasma glutathione and in-vitro sperm nuclear decondensation (NCDT).Methods and Material: the present study was done on 80 male patients of 21-58 yrs of age which were divided into three groups i.e. Group I: Controls- fertile men (n=30) ; Group II: Normozoospermic infertile men (n=30) ; Group III: Oligozoospermic infertile men (n=20). Levels of the reduced glutathione was measured and spermatozoa nuclear chromatin decondensation test (NCDT) was done. These two parameters were then correlated. Statistical analysis used: ANOVA test and Bonferroni’s post test. Coefficient of correlation (r- value) was found to find relationship between different parameters.Results: reduced glutathione level (GSH) and NCDT score was significantly more in group 1 as compared to the other groups. Coefficicent of correlation between reduced glutathione level (GSH) and NCDT score 0.62, 0.57and 0.71 respectively in group 1, group 2 and group 3. Conclusions: the ability of the sperm chromatin to undergo decondensation following fertilization is affected by reduced glutathione level in seminal plasma.