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Albumin improved spermatozoa quality and dna integrity for freezing-free preservation

Authors:K Osman, CF Nang, SF Ibrahim, SB Budin, FHF Jaffar, NAA Wahab
Int J Biol Med Res. 2012; 3(2): 1670 – 1679  |  PDF File


Freezing-free preservation provides a simple yet efficient alternative to semen preservation. Our study objectives were to determine optimum preservation temperature and bovine serum albumin (BSA) concentration for spermatozoa storage. It was conducted in two phases. First phase involved storage temperature optimization through viability assessment. Temperatures used for this phase of the study were 4, 25 and 37 C. The second phase involved the use of identified optimized temperature in determining optimum BSA concentration. This is achieved through the effect of BSA on viability and DNA integrity. Concentrations that were used to achieve this objective were 0, 1, 4, 8, 12, and 16 mg/ml BSA. Our results showed that optimum temperature was at 40C. By using ANOVA analysis, our optimum BSA concentration for improving viability and DNA integrity were at 8 mg/ml BSA. Multiple regressions test showed that viability percentage can actually be predicted by storage temperature and time using this equation model: Viability = (-0.839)(Time) + (-0.269)(Temperature) + 44.806. Our study had also indicated that for bovine spermatozoa stored at 40C, prediction on viability was influence solely by storage time regardless of BSA concentration, with Viability = (-0.612)(Time) + 33.752. Bovine spermatozoa DNA integrity, however, can be predicted by both BSA concentration and storage time, with Spermatozoa DNA Damage = (0.714)(Time) + (-0.161)[BSA] + 2.411. In conclusion, optimum freezing-free preservation temperature is at 4C with 8 mg/ml BSA supplementation.