Speciation of candida by hicrome agar and sugar assimilation test in both hiv infected and non infected patients.

Authors:Veena Manjunath, Vidya GS, Archana Sharma, Mridula Raj Prakash, Murugesh
Int J Biol Med Res. 2012; 3(2): 1778 – 1782  |  PDF File

Abstract

Background: Candida spp. are the fourth most common pathogens isolated from blood cultures and the numbers of non-albicans Candida(NAC) with decreased susceptibility to anti-fungal agents are also increasing. Rapid identification of yeast isolates to the spp. level is essential in order to optimize the antifungal treatment. Aim: To speciate the isolates obtained from both HIVinfected and non-infected patients using chrom agar and to carryout the antifungal susceptibility by disk diffusion method and also to detect the common candidial species in HIVinfected patients in our hospital. Materials and Methods: A total of 150 samples were collected from both HIV infected and non infected patients and were subjected to culture on Chromagar , germ tube test, sugar assimilation test and their antifungal susceptibility for fluconazole and voriconazole. Results: A total of 136 isolates were obtained from 150 samples. Of which 57 were from HIV infected and 79 from patients with suspected candidiasis. C.albicans was the most common isolates in both the study groups. Among non-albicans candida in HIV infected patients, C.glbrata was the next common isolate seen whereas in the non infected patients it was C.tropicalis. They all showed increased resistance to fluconazole but were sensitive to voriconazole except for one glabrata isolate. C.albicans in both (HIVinfected and non-infected) the groups showed 98% and 96% sensitivity to voriconazole respectively but its resistance pattern to fluconzole was noted more in case of HIV infected patients.