Antioxidant effect of gycowithanolides on esterase activity in salivary glands of d-galactose stressed mice

Authors:Radhika Nandkishor Mote, MM Pillai, BK Pawar
Int J Biol Med Res. 2010; 1(4): 193-201  |  PDF File

Abstract

Aging is inevitable process, characterized by diminishing functional capacity of an organism so it fails to cope up with stress. Varieties of antioxidants are suggested by researchers to reduce the adverse effects of aging. The present study was undertaken to assess the antioxidant effect of glycowithanolides, extracted from Ashwagandha (Withania somnifera) leaves on esterase activity in submandibular and sublingual salivary glands of D-galactose induced aging mice. Adult male mice (Mus musculus) of 5 to 6 months age were divided into main two group viz. protective group and curative group. Each group further divided into four batches viz. 1) Control batch of protective and curative group received 0.5 ml 0.9% saline per day for 20 days and 40 days respectively. Mice from 2nd, 3rd and 4th batches of protective group received D-galactose, D-galactose +centrophenoxine (CPH) and D-galactose + Glycowithanolides (WSG) for 20 days respectively. Mice from 2nd, 3rd and 4th batches of curative group received D-galactose for 20 days followed by saline, CPH and WSG alone for further 20 days respectively. In D-galactose induced aging mice esterase activity was reduced significantly in both, submandibular and sublingual salivary glands compared to the control batch. In CPH and WSG treated mice there was significant increase in esterase activity compared to the D-galactose stressed mice. During aging stress induced by D-galactose alterations in esterase activity of salivary glands took place. These alterations were prevented and cured by CPH and WSG. Esterase activity was increased significantly in WSG treatment than CPH. Protective effect was more pronounced than curative effect.