Adhesion force of t lymphocytes in the pathogenesis of patients with light and severe bronchial and atopic asthma

Authors:Ckibo Y.V, VodounoN A.C , Морозов M.B , Koub Bocco S.M , Sina H , Kurmaeva N , Tsibulkina V. N, Kotchoni Siméon O , Baba-Moussa Lamine , Abramova
Int J Biol Med Res. 2012; 3(3): 2138-2146  |  PDF File

Abstract

The emergence of various diseases specifically disabling diseases such as bronchial and atopic asthma is associated with quantitative and qualitative changes of lymphocytes. Therefore, the detection in the peripheral blood of some diseases, the so-called degenerative lymphocytes might constitute a new diagnostic and prognosis test and might serve as markers, and even constitute innovative therapeutic targets. Atomic force microscope, considered as a new method of investigation, allowed us to visualize the structure of lymphocytes at a degree of molecular resolution, to study the spatial distribution of mechanical properties of cellular structures within a single cell and the adhesive force of cell surface of lymphocytes in light and severe asthmatics. The results showed that the more the inflammatory process is high the more important the cell adhesion. With Atomic Force Microscope method a relationship of interdependency between morphological changes and the degree of severity was established. These morphological changes might lead to the increase in the force of adhesion, the release of lipidic mediators, the lymphokines capable of activating the surrounding cells and to boost the allergic inflammation. The images obtained on the morphology of patient lymphocytes could suggest a variability of morphological parameters of cells according to the severity of the disease. But these parameters vary significantly within each group. This suggests that the emergence and intensification of the inflammatory process are not related to changes in cellular parameters but rather the functioning of lymphocyte involved in the activation of immuno-inflammatory system. Therefore, using the Atomic Force Microscope method in the field of biomedical science can lead significantly to go beyond the study of morphological parameters of human blood cells and especially apoptotic lymphocytes.