Inflammatory responses in liver induced by high fat plus fructose diet: therapeutic potential of cissus quadrangularis stem

Authors:Chidambaram Jaya, Vetriselvi Venkatesan­, Carani Venkatraman Anuradha
Int J Biol Med Res. 2010; 1(4): 120 – 124  |  PDF File

Abstract

There is evidence that high fat-fructose diet (HFFD) induces hepatic insulin resistance (IR), fatty liver, and oxidative stress in rats. The purpose of this study is to evaluate the inflammatory changes in HFFD-fed rat liver and the effect of Cissus quadrangularis stem extract (CQEt) on IR and inflammation in these rats. Rats were given HFFD for 60 days. A group of HFFD-fed rats were administered CQEt from the 15th day of the experimental period till the end of the study. HFFD-fed animals displayed decreased glucose/insulin (G/I) ratio and insulinogenic index (30 minutes) indicating the development of IR. CQEt addition significantly improved the insulin sensitivity. Total liver RNA was isolated for real-time PCR (RT-PCR) analysis. Expression of genes related to inflammation and fibrosis such as tumour necrosis factor (TNF-α) and transforming growth factor (TGF-β1) were synergistically increased in the HFFD- fed rats while CQEt-treated HFFD-fed rats showed significant decrease in the expression of these genes. In addition, HFFD rats displayed increased expression of α-smooth muscle actin (α-SMA) and TGF-β1 as seen by immunohistochemistry. CQEt treatment downregulated the expressions of α-SMA and TGF-β1. The findings of the study provide new data that CQEt markedly reduces diet-induced hepatofibrogenesis by controlling the activation of inflammatory and profibrogenic genes.