Synergistic activity of chloroform extract of durio zibethinus wood bark with penicillin g against staphylococcus aureus

Abstract

Aim: This study is conducted to determine the synergistic activity of chloroform extract of Durio zibethinus wood bark with Penicillin G against Staphylococcus aureus. Method:Durio zibethinus wood bark extract was obtained through the process called maceration, filtration,evaporationand the powder form was freshly reconstitute in dimethyl sulfoxide (DMSO) and tested against Staphylococcus aureus ATCC 33862 using Kirby Baur technique and the plates were incubated at 37 ºC. The zone of inhibition was measured after 24 hours and recorded in millimeters. The synergistic study was conducted using the Durio zibethinus wood bark extract in combination with penicillin G with the propotion of 1:1 in homogenous condition and incubated at 37 ºC for 24 hours. The zone of inhibition was measured and recorded. Result: The inhibition zone of Durio zibethinuswood bark extract against Staphylococcus aureus ATCC 33862 was 10 mm and the Penicillin G was 12 mm. The combination of both Durio zibethinus wood bark extract and Penicillin G was 14 mm. Conclusion: Staphylococcus aureus have created resistance to various antibiotics. Chloroform extract of Durio zibethinus bark exhibit mild synergistic activity against Staphylococcus aureus ATCC 33862. Although the effect is mild but it is possible that Duriozibethinus could reverse such resistance and thus potentiate the effect of common penicillins against resistant Staphylococcus aureus.