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Detection of esbl producers from pus samples of diabetic skin and soft tissue infections

Authors:Saroj Golia , Vivek Hittinahalli , K Mallika Reddy ,K Sujatha Karjigi
Int J Biol Med Res. 2013; 4(1): 2732-2735  |  PDF File

Abstract

Objective—To determine the microbiological profile and antibiotic susceptibility patterns of organisms isolated from diabetic skin and soft tissue infections and detection of ESBL producing Gram negative organisms. Material and methods—Pus samples for bacterial culture were collected from 70 patients admitted with diabetic skin and soft tissue infections. The isolates were tested for antimicrobial susceptibility by disc diffusion technique according to clinical and laboratory standards institute (CLSI) guidelines. The screening for ESBL production in Gram-negative bacilli was done by using ceftazidime and cefotaxime discs as recommended by CLSI for extended spectrum β-lactamase (ESBL) production and suspected ESBL producing organisms are confirmed by double disc approximation method. Results. A total of 119 organisms were isolated from 70 patients, among these 40 were gram positive organisms, 67 were gram negative and 12 were candida spp. Among gram negative bacilli klebsiella species were highest ESBL producer(63%), followed by Pseudomonas Species(39%). All the ESBL-producing isolates were found to be 100% sensitive to imipenem. Conclusions—. The prevalence of ESBLS among members of Enterobacteriaceae is a serious threat, leading to treatment failure and complications. There is an urgent need to emphasize rational use of drugs to minimize the misuse of available antimicrobials. There is a need for continuous surveillance of resistant bacteria to provide the basis for empirical therapy and reduce the risk of complications.