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Screening and characterization of microorganisms capable of producing antineoplastic drug, l-asparaginase

Authors:Yogendra Singh, S. K. Srivastava
Int J Biol Med Res. 2012; 3(4): 2548-2554  |  PDF File


In recent years, microbial asparaginases have drawn particular interest because of their potential antineoplastic properties and significant application in food industries. The present survey was undertaken to establish other microbial species which produce L-asparaginase, possibly in more effective and specific form, and which might have even more efficient antineoplastic action.15 soil isolates and 12 other microorganisms from different culture collections were evaluated for their L-asparaginase producing ability. The activity was identified on a agar medium containing 3.0 gL-1 glucose, 6.0 gL-1 Na2HPO4.2H2O, 3.0 gL-1 KH2PO4, 0.5 gL-1 MgSO4.7H2O, 0.5 gL-1 NaCl, 0.015 gL-1 CaCl2.2H2O, 3.0 gL-1 L-asparagine at and phenol red. Phenol red was used as an indicator for the production of enzyme L-asparaginase. Among all the microorganisms tested, four organisms from soil sample and nine cultures from various culture collections in India showed their potential for the enzyme production. Further morphological and biochemical characterization resulted that the soil isolates were belonging to the genera Bacillus spp., Proteus spp. and Pseudomonas spp. The strain, designated as ITBHU02, has shown maximum activity of enzyme L-asparaginase.