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Characterization of antioxidant profile of human saliva during menstrual cycle

Authors:Subbarayalu Alagendran, Govindaraju Archunan, R.L.Rengarajan, S. Muthukumar, R. Ilayaraja, A. Amarnat, Balamuthu Kadalmani, Gabriella Fernandez, Rosalinda Guevara Guzman
Int J Biol Med Res. 2011; 2(1): 382 – 389  |  PDF File


The aim of this study was to determine the relationship of antioxidants profile with endogenous reproductive hormone level across the menstrual cycle. Predicting ovulation is the basis on which the fertile period is determined. Currently there are many methods accessible to identify the ovulatory period. Unfortunately, these methods are not always efficient for accurate detection of ovulation. Hence, an attempt was made to detect ovulation through antioxidant profile with the help of BBT, saliva ferning and ultrasound. Salivary antioxidants were investigated serially during preovulatory, ovulatory and postovulatory periods of normal menstrual cycle in twenty healthy volunteers. The sample was collected in three consecutive menstrual cycles. Salivary antioxidants was estimated and analysis by UV-spectrophotometer. The results revealed higher significant variations in the antioxidant concentration and physiological changes are also essential owing to ovarian hormones during menstrual cycle. Alkaline phopshatase, peroxidase, ascorbic acid and lactate dehydrogenase were maximum during ovulation and minimum during postovulatory phase. Superoxide dismutase, catalase and glutathione peroxidase were decrease in phases of menstrual cycle. Among these antioxidants alkaline phopshatase, peroxidase and ascorbic acid were predominantly exhibited during ovulatory phase than pre and post-ovulatory phase. Therefore, we studied the effects of 17 β-oestradiol and progesterone on the antioxidative status and the vulnerability to oxidative stress exhibited during normal menstrual cycle.