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High performance thin layer chromatographic method for estimation of erlotinib hydrochloride as bulk drug

Authors:Valluru Rajesh, B.Anupama, V.Jagathi, K.Varaprasad
Int J Biol Med Res. 2011; 2(1): 433 – 435  |  PDF File

Abstract

ABSTRACT: A simple, specific and precise high performance thin layer chromatographic method was developed and validated for estimation of Erlotinib hydrochloride as bulk drug. The chromatographic development was carried out on precoated silica gel 60 F254 aluminium plates using mixture of Methanol: Ammonia (8:0.2 v/v) as mobile phase and densitometric evaluation of band was carried out at 250 nm using Camag TLC Scanner-3 with win CAT 1.4.3 version software. The Rf value of drug was found to be 0.52 ± 0.01. The method was validated with respect to linearity, accuracy, precision and robustness. The calibration curve was found to be linear over a range of 200- 1200 ng/ band. The % assay (Mean ± S.D.) was found to be 101.3 ± 1.02. Thus the proposed HPTLC method was found to provide a faster and cost effective quantitative control for routine analysis of Erlotinib hydrochloride as bulk drug.