Comparative study of dna fingerprinting of biological samples obtained from indian buffaloes by using rflp, aflp and rapd methods

Abstract

Random Fragments Length Polymorphisms (RFLP) by using 14 samples from both breeds (Murrah and Surti buffalo) with the enzyme (EcoR-1), the genetic material of the same breed animals were more similar, a though not entirely unanimous but the different genetic backgrounds of these two species generated different fingerprint patterns. While, the AFLP fingerprinting technique by using two primers (EcoRI FP and EcoRI RP) with 21 samples of (Murrah and Surti breeds), the results showed similar within breed and different between two breeds. The different genetic backgrounds of these species generated different fingerprinting patterns between breeds, and similar fingerprinting patterns within breed. The last technique RAPD-PCR, were applied to study the genetic distance and genetic similarity in two Indian buffaloes breeds Murrah and Surti with a battery of 16 primer. RAPD pattern revealed the total polymorphism loci of two breeds ( 131 loci), the total loci amplified in two breeds (169). The estimate of genetic distance was highest (2.30) with the primer KMS1 and the lowest (0.41) with the primer KMS12. The GD pooled over the primers was (1.41±0.05) between these two breeds. The highest percentage difference estimate (100) with the primer KMS7 and the lowest (25) with the primer KMS15 and the MAPD between two breeds estimate (62.9±0.9).