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Effects of genistein and daidzein, in combination, on the expression pattern of biomolecular markers (p53, PCNA, VEGF, iNOS, Bcl-2, and Bax) during 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in sprague-dawley rats

Authors:Pachaiappan Pugalendhi, Shanmugam Manoharan, Nagarethinam Baskaran, Madhavan R. Nirmal
Int J Biol Med Res. 2010; 1(4): 264-271  |  PDF File

Abstract

Evaluation of molecular markers in the mammary tissues has prognostic and therapeutic values. Aim of the present study was to evaluate the effects of genistein and daidzein, in combination, on the expression pattern of biomolecular markers such as p53, PCNA, VEGF, iNOS, Bcl-2, and Bax during DMBA induced mammary carcinogenesis in Sprague-Dawley rats. Mammary tissues were routinely processed and paraffin embedded, 2-3µm sections were cut in a rotary microtome and mounted on clean glass slides. Immunohistochemical staining of mammary tissue was performed and each slide was microscopically analyzed and enumerated the percentage of the positively stained cells semi-quantitatively and scoring was made. Immunohistochemical analysis revealed over expression of p53, PCNA, VEGF, iNOS, Bcl-2 and decreased expression of Bax in the mammary tissues of DMBA treated rats. Oral administration of genistein+daidzein (20mg+20mg/kg bw) in combination, to DMBA treated rats significantly down regulated the expression of p53, PCNA, VEGF, iNOS, Bcl-2, and up regulated the expression of Bax proteins. Our results suggest that the pleiotropic and synergistic effects of genistein and daidzein might have reduced cellular proliferation, decreased angiogenesis and induced apoptosis during DMBA induced mammary carcinogenesis in Sprague-Dawley rats.