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Adiponectin/leptin ratio as a biomarker of acute metabolic stress

Authors:Elshaari, FA, **Alshaari AA, Sara Ali, Hassan SA , Sheriff DS
Int J Biol Med Res. 2013; 4(3): 3278-3283  |  PDF File

Abstract

Obesity is a major problem for Libya. The need for research and ways to tackle obesity related problems initiated us to undertake an experimental approach utilizing mice wherein we tried to create an acute metabolic stress using high fat diet which does not precipitate obesity. The mice were fed with high fat diet for 3 weeks and 7 weeks period respectively where histological changes in liver indicate the presence of steatosis without marked changes in body weight. Fasting blood glucose level, serum lipid profile and the levels of adiponectin and leptin were measured. The presence of oxidant stress is evaluated by estimating liver glucose-6-phophsate dehydrogenase activity, glutathione (G.SH) levels and thiobarbutric acid reactive products (TBAR) measured as MDA levels. Adiponectin /Leptin ratio is taken as a marker to evaluate the effect of metabolic stress. It was observed that there were no marked changes in body weight of the experimental animals. The serum levels of adiponectin was increased significantly in 3 and 7 weeks duration with a possible increase in the levels of leptin in the 7 week rodents with impaired glucose tolerance, dyslipidemia and insulin resistance. The ratio between adiponectin and leptin remained higher during the acute phase of metabolic stress as evidenced in the 3 weeks group when compared to 7 week group which showed a significant reduction. It appears therefore, adiponectin/leptin ratio could be a better biomarker of acute metabolic stress.