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Evaluation of endothelial and platelet functions with intergenotypic variation of eNOS Glu298Asp gene polymorphism in relation to postmenopausal women

Authors:Pradeep Kumar Dabla, Sarika Arora, Shubha Sagar Trivedi, Nibhriti Das, Jayashrees Bhattacharjee
Int J Biol Med Res. 2010; 1(4): 272-276  |  PDF File


Menopause is associated with increased Coronary Artery Disease (CAD) risk. This study was planned to elucidate the endothelial changes and platelet dysfunction and intergenotypic variation of NO levels in eNOS Glu298Asp gene polymorphism in postmenopausal women. This study was done on 200 women in the age group 40-55 years were selected from a tertiary care hospital. Of these, 100 postmenopausal women were included in study group and 100 premenopausal women served as control. Fasting blood samples were evaluated for plasma estriol, Nitric Oxide (NO) and Platelet Factor-4 (PF-4) levels. PCR-RFLP was done in remaining cell aggregate to study eNOS Glu298Asp gene polymorphism. Statistical evaluation was done on SPSS. Postmenopausal women had significantly lower estriol and NO levels as compared to premenopausal women (p<0.001). Significant positive correlation was found between age with PF4 in study group (p<0.01) and NO with estriol in both groups whereas negative correlation was observed between NO with PF4 in both groups. Distribution of genotype GG, GT and TT in postmenopausal and premenopausal women was found to be 82, 18, 0% and 90, 10, 0% respectively. All genotypes (GG+GT, GG and GT) showed significant decrease (p<0.001) in NO level in postmenopausal women. Decreased estrogen levels in postmenopausal state lead to endothelial and platelet dysfunction due to low NO levels. Further presence of eNOS gene polymorphism may provide additional insult by decreasing NO levels adding to the CAD risk. Hence, postmenopausal women those who presents with eNOS Glu298Asp polymorphic gene may have higher CAD risk.