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Detection of extended spectrum beta-lactamase producing gram negative bacilli in urinary isolates

Authors:Rajesh Kondian Rangachari , Mathavi Suresh Kumar, Indra Priyadarsini
Int J Biol Med Res. 2010; 1(4): 130-132  |  PDF File


Background Production of Extended Spectrum Beta-Lactamase is a kind of antimicrobial resistance exhibited by bacteria which is a chronic problem in a health care set up. Obtaining knowledge about the prevalence of Extended Spectrum Beta Lactamase producing organisms would guide in therapeutic management of infections in a given environment. Aim & Objectives The purpose of the study was to detect Extended Spectrum Beta-Lactamase producing Gram Negative Bacilli in Urinary isolates from a tertiary care hospital. Material & Methods A total of 482 non-repetitive Urine samples were collected over a period of six months (Apr. 2010 to Sept.2010). The Gram Negative bacilli isolates that were resistant or with lesser susceptibility to any of the third generation cephalosporins were selected for the study. Phenotypic confirmation of Extended Spectrum Beta-Lactamases production was done by Double Disc Synergy Test in which the zone size around the third generation cephalosporins antibiotic increased towards the Augmentin disc. Results In our study among the 129 Gram Negative Bacilli isolated from urine, 29 (22.48 %) were positive for ESBL by Double Disc Synergy Test (DDST). Conclusion ESBL was found to be produced by 22.48 % of our isolates. Proper antibiotic policy and appropriate guidelines to prescribe antibiotics are the routes to prevent dissemination of Multidrug Resistant Organisms.