Differentiation and identification of biological samples obtained from indian cattle using dna fingerprinting technology (minisatellites

Abstract

Minisatellites or VNTR fingerprinting of 20 samples of Holstein and Jersey cattle breed amplified with (FP) and (RP) primers, the results showed that the varied VNTR patterns were generated from genomic templates depending on the primers and species. The same primer applied to the same breed, all the main bands are the similar but which were different among species, which is lead to the different fingerprinting patterns. Whereas, AFLP-PCR showed that the patterns of DNA fingerprinting amplified with EcoRI FP and EcoRI RF were very different among species of Holstein and Jersey cattle breeds, but similar within specie. Different genetic backgrounds of these two species generated different fingerprint patterns. The last technique RAPD-PCR was carried out with 9 random primers. From the 9 random primers, a total of 49 bands were amplified and 33 of these ( about 66.87% ) were found to be polymorphic among the Holstein population and a total of 53 bands were amplified out of which only 35 were polymorphic giving (about 66.20%) polymorphism in Jersey cattle. The estimate of GI was highest (0.679) with the primer OPS-13 and the lowest (0.139) with the primer OPS-06. The GI pooled over the primers was (0.309±0.06) between these breeds and the MAPD estimate to be (66.9866±6.34). It might be concluded that the genetic identity index between these two breeds on the basis of BF showed moderate level of GI with the primers employed. MAPD calculated on the basis of uncommon bands also demonstrated lower to medium level of genetic difference between Holstein and Jersey breeds of cattle.