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Vaccine development against the matrix gene (m-protein) of influenza virus type-a, subtype-h5n1

Authors:Ali Nayyef Umayra, Harish Kumar Bajaj
Int J Biol Med Res. 2013; 4(3): 3360-3363  |  PDF File


Influenza is one of the prominent diseases of interest to the research domain because of its constant changes in the antigenic property of the virus. Therefore there is a need to develop strain specific influenza vaccines. Current work is on the study of pathogenicity of H5N1 strain in causing Avian Influenza (AI) and the vaccine development against the disease using the reverse vaccinology approach. To study the pathogenicity of the disease various assays were performed using Immuno techniques like Agglutination& Precipitation tests. The Matrix Protein of Influenza virus is of high consideration and has been reported to play a prominent role in creating pathogenicity. The matrix2 protein was taken into consideration for our study because this protein was found to be conserved in many influenza strains. DNA Isolation was done from the avian influenza infected blood samples of human and chicken. The matrix2 (M2) gene sequence is amplified using (PCR) Technique and sequenced. The work was then extended towards development of subunit vaccine (peptide vaccine) using the Reverse vaccinology approach of bioinformatics. The antigenicity studies, epitopic prediction and surface accessibility area calculation are steps required for deciding the best antigenic peptides. All the above analysis is performed for the matrix2 protein of human and chicken. The antigenic peptides were designed and secondary structure prediction was done. Based on the energy of the structure we can predict the best antigenic peptide which has the capability of being a potent peptide vaccine against Influenza A virus subtype H5N1 which cause a zoonoticdisease called Avian Influenza (AI).