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Bacteriological profile along with special reference to rapid diagnosis of group b streptococcal septicemia in neonates

Authors:MD.Khalid Rashid*, Amar C.Sajjan,Ved prakash
Int J Biol Med Res. 2014; 5(2): 3991-3993  |  PDF File

Abstract

Aim: to find out the bacteriological profile of neonatal sepsis with special reference about incidence of group B streptococci and its rapid diagnosis by detecting antigen in urine. Material and methods: 70 selected neonates having clinical manifestation of sepsis were selected for the study .Blood and urine was collected under aseptic condition .of these patients 30 urine samples were subjected to a commercially available latex particle agglutination assay for detection of group B streptococcal antigen. Blood cultures were done on all these samples for finding out the bacteriological profile in neonatal septicemia. Results: Blood culture was positive in 33(47%) cases ,sterile in 30(40%) cases, and contaminated in 7(10%) cases. Majority of predominant isolates belong to family enterobacteriaceae,with predominance of Klebsiella pneumonia 10(33.3%), Escherichia coli 5(15.01%),Staphylococcus aureus 5(15.01%),Acinetobacter spp 5(15.01%),Coagulase negative staphylococci 5(15.01%) .Group B streptococci was isolated in only 1(3.03%) case. Group B antigen was detected in 2 urine specimens. Conclusion: Members of family enterobacteriaceae are the predominant isolate in cases of neonatal sepsis in our country. A group B streptococcus is not a common pathogen in neonatal sepsis in our country. Detection of group B streptococci by antigen detection using latex particle agglutination assay is a very simple, rapid, commercially available assay with a high degree of sensitivity and specificity.