IC Value
Search
About Us
Authors
Reviewers
Editorial Board
Contact Us

Assessments of molecular and biochemical analysis of the chemotherapeutic drugs adriamycin, cisplatin and fluorouracil on serum of male albino rat

Authors:Karolin K Abdul-Aziz
Int J Biol Med Res. 2014; 5(4): 4499-4504  |  PDF File

Abstract

Objective: The aim of the present investigation was to evaluate the cyototoxic effect of commonly used neoplastic drugs including adriamycin, cisplatin and 5-FU. Methods: Twenty-four mature male albino rats weighing approximately 130-150 g body weight were arranged into four groups; control, adriamycin (0.2 mg/kg.b.wt), cisplatin (0.2 mg/kgb.wt.) and fluorouracil (20 mg/kg. b.wt.). The applied dose was intraperitoneally injected every other day for 21 days. Isoenzyme electrophoresis of alkaline-acid phosphatase, lactic dehydrogenase and glucose 6-phosphate dehydrogenase were investigated using electrophoresis. As well as, plasma content of vascular endothelial growth factor (VEGF), heat shock protein 70 (HSP-70), troponin-t, 8-hydroxy-2′-deoxyguanosine (8-OHdG), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was measured by ELISA. Results: Different chemotherapeutic treatments exhibited alterations of the activity of isoenzymes including acid phosphatase, lactic dehydrogenase and glucose 6 phosphate dehydrogenase. As well as a significant reduction in serum contents of VEGF, HSP-70, bICAM-1 and VCAM-1, in contrary, an increase in troponin-t and 8-OHdG were observed. The present findings concluded that chemotherapeutic treatment interfere with cell function causing alterations in isoenzymes and reduction of cell growth by decreasing of VEGF, HSP-70 and cell adhesion molecules. As well as predict of cell damage as detected by increase of serum troponin-t and 8-OHdG.