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Prevalence of different virulence factors and biofilm production ability of urinary escherichia coli isolates

Authors:Motahareh Samet,Ezatallah Ghaemi, Mesam Hassan Nejad, Ailar Jamali
Int J Biol Med Res. 2014; 5(4): 4546-4549  |  PDF File

Abstract

One of the initial steps of uropathogenic Escherichia coli (UPEC) to establish the urinary system and causes urinary tract infection (UTI) is biofilm formation. Several genes known to affect biofilm formation. The aim of this study was assess the ability of biofilm formation and frequency of the three effective genes of urinary E. coli isolates in Gorgan. A total of 170 isolates of urinary Escherichia coli were collected and biofilm formation was investigated with microtiter plate method in BHI medium containing 1% sucrose. Prevalence of Curli adhesin, Antigen 43 and extracellular polysaccharide Colanic acid genes were investigated with PCR methode and using specific primers. Statistical comparison of data was performed using the x2 and P <0.05 was considered significant. 105 (61.8%) UPEC isolates could form biofilm. Isolates without colanic acid, were not been able to form biofilms. Prevalence of csgA, flu and wca genes were 156 (91.8%), 127 (74.7%) and 160 (94.1%), respectively. In the isolates able to form biofilm, the frequency of csgA, flu and wca genes were 99%, 97.1% and 100%, respectively. This difference is statistically significant (P<0.001). In conclusion our finding demonstrated that three factors (Ag43, Colonic Acid and Curli) are essential for biofilm formation. In this study although over 60% of UPEC isolates were able to form biofilms.