The role of m235t angiotensinogen gene polymorphisms in the occurence of acute ischemic stroke in hypertensive subjects

Abstract

Ischemic stroke is highly related with hypertension. Other risk factors such as smoking, diabetes and hypercholesteremia also play important roles. Recent findings suggest that various types of gene polymorphisms may also contribute to the risk of stroke. While gene polymorphism for angiotensinogen (AGT) is reported to contribute to the regulation of blood pressure, its effect on the risk of acute ischemic stroke remains controversial. We hypothesized that polymorphism of the AGT gene could be a risk factor for acute ischemic stroke in hypertensive subjects. This case-control study took place in Wahidin Sudirohusodo General Hospital, Makassar, Indonesia. All cases subject (hypertensive subjects with acute ischemic stroke) and controls subject (hypertensive subjects without acute ischemic stroke) were took the blood sample to examined M235T AGT gen polymorphism as well as glucose blood, lipid profile, and also smoking history as risk factors. The result showed that variables such as age, gender, and smoking status show no difference between case and control group (p > 0.05). However, diabetes mellitus and dyslipidemia is more likely in case group than control group (p <0.05). As risk factor for acute ischemic stroke, the TT and MT genotype M235T AGT gene polymorphism has OR=6,4 and OR = 1,3 respectively, against MM genotype. TT genotype M235T AGT gene polymorphism, diabetes mellitus and dyslipidemia together as risk factors accounted 32.1% for acute ischemic stroke event in this study, with OR of 20.5 for diabetes mellitus; 10.9 for the TT genotype of AGT M235T gene; and 4.8 for dyslipidemia. In conclucion, we found that TT genotype M235T AGT gene polymorphism maybe a risk factor of acute ischemic stroke in addition to diabetes mellitus and dyslipidemia.